HPV18 DNA levels are not prognostic for precancerous cervical lesions

[Momtezuma Tuatara]

http://www.eurekalert.org/pub_releases/2009-01/jotn-hdl012309.php

Public release date: 27-Jan-2009
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Contact: Caroline McNeil
jncimedia@oxfordjournals.org (jncimedia@oxfordjournals.org)
301-841-1287
Journal of the National Cancer Institute (http://jncicancerspectrum.oupjournals.org/)

HPV18 DNA levels are not prognostic for precancerous cervical lesions



Perhaps surprisingly, the number of copies of the carcinogenic human papillomavirus type 18 (HPV18) relative to cellular DNA is not associated with the likelihood of progression to advanced precancerous lesions of the cervix, according to a study in the January 27 online issue of the Journal of the National Cancer Institute.

Two types of HPV are most frequently associated with cervical cancer, HPV16 and HPV18. Previous studies showed that the number of HPV16 copies per cell correlated with an increasing risk of progression to cervical intraepithelial neoplasia grade 2 or 3 (CIN2-3). The prognostic significance of HPV18 DNA level is not known.

In the current study, Long Fu Xi, M.D, Ph.D., of the University of Washington in Seattle, and colleagues compared the number of copies of HPV18 DNA relative to cellular DNA at baseline with a woman's risk of progressing to CIN2-3. The 303 study participants were drawn from the Atypical Squamous Cells of Undetermined Significance and Low-Grade Squamous Intraepithelial Lesion Triage Study.

During the 2-year study period, 92 women were diagnosed with CIN2-3. Among women with a cytologic diagnosis of low- or high-grade squamous intraepithelial lesions at enrollment, HPV18 DNA level was lower in women with CIN2-3 than those without CIN2-3.

"In summary, our data indicated that HPV18 DNA levels were highest among women with evidence of a benign squamous intraepithelial lesion, intermediate among those with CIN2-3, and lowest among those with normal cytological findings," the authors write. "Thus, testing for high levels of HPV18 DNA does not appear to be clinically useful."

In an accompanying editorial, Eduardo Franco, Dr.P.H., and François Coutlée, M.D., of McGill University in Montreal, agree with the authors' conclusion and applaud their effort to elucidate the underlying biology of cervical cancer. "As far as clinical practice is concerned, the obvious conclusion from the study of Xi et al. is that quantifying the HPV18 DNA load may not have the same value as for HPV16," the editorialists write.

"That said, the findings from this study considerably extend our appreciation for the heterogeneity of molecular events and their cellular targets in cervical carcinogenesis."

[Momtezuma Tuatara]

Pub med citation: (http://www.ncbi.nlm.nih.gov/pubmed/19176455?dopt=Abstract)

J Natl Cancer Inst. (javascript:AL_get(this, 'jour', 'J Natl Cancer Inst.');) 2009 Jan 27. [Epub ahead of print]
Related Articles (http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&DbFrom=pubmed&Cmd=Link&LinkName=pubmed_pubmed&LinkReadableName=Related%20Articles&IdsFromResult=19176455&ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Links (javascript:PopUpMenu2_Set(Menu19176455);)
(http://www.ncbi.nlm.nih.gov/entrez/utils/fref.fcgi?PrId=3051&itool=Abstract-def&uid=19176455&db=pubmed&url=http://jnci.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=19176455)
Prognostic Value of Measuring Load of Human Papillomavirus DNA in Cervical Samples: An Elusive Target.

Franco EL (http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Franco%20EL%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract), Coutlée F (http://www.ncbi.nlm.nih.gov/sites/entrez?Db=pubmed&Cmd=Search&Term=%22Coutl%C3%A9e%20F%22%5BAuthor%5D&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsP anel.Pubmed_RVAbstract).

Affiliations of authors: Department of Oncology (ELF, FC) and Department of Epidemiology and Biostatistics (ELF), McGill University, Montreal, Quebec, Canada; Laboratoire de Virologie Moléculaire, Centre de Recherche and Département de Microbiologie-Infectiologie, Centre Hospitalier de l'Université de Montréal, Montreal, Quebec, Canada (FC).

PMID: 19176455 [PubMed - as supplied by publisher]
this is the full article here: http://jnci.oxfordjournals.org/cgi/reprint/101/3/131

[magical1]

Hilary could you clarify something for me?

When one gets an abnormal smear why does no one ever do a HPV type test or an HPV test?

I asked my gyne for one and she said they don't do them. I always was under the impression that you could have them but they cost about $400.00

How do I even know that I have HPV? at all? No one has ever tested me for it.

Do the people that actually get cancer get tested for the type of HPV they have?

[Momtezuma Tuatara]

Not as far as I know. But also, as I said in my second book, the currently used tests for HPV virus don't pick up at least 70% of them, so what would be the point anyway?

[magical1]

Well I guess the point would be that if we apparently get cancer only from 16 and 18 HPV how on earth do they track this if no one is getting the tests??

[Momtezuma Tuatara]

They don't. And there are at least 30 different HPV viruses they say can "cause" cancer, and every time they look, they find new varieties they never knew existed before.

http://www.fda.gov/cber/minutes/0910evolv.txt (http://www.fda.gov/cber/minutes/0910evolv.txt)

Quote:
Pg 84-85 ~ I saved it to a word document

It is known that these patients all have particular cell-mediated
immune deficiencies. Again, suggesting that particular arms of the immune
system are responsible for either containing or failing to contain different
subgroups of the papilloma viruses. As we look at these women over a period
of time through these six month or so samples, what we also find, and other
labs have exactly the same results, is every time we sample, you may or may
not see the type you saw before. It may switch. For (Pg 85) instance, we
have this patient who had 6 plus 16, and then 11 plus one that was minor and
we couldn't tell, then jyn 2, and then type 40, and then we had a type 4,
but the others disappeared.

(So does HPV do turn about and hide in the body? If so, how would you know what the body has contacted and what it hasn't?)

Page 87-88

I feel that they are virtually ubiquitous. they are typically
sub-clinical, persist in or latent (pg 88) infections. There are
staggeringly large number of genotypes if we take the care to look. I might
say that the reason these are typically not found is that people use generic
cross-hybridizing probes or have cut off their probe sets. If you're not
probing for something, you are not going to see it.

My Page 88 and 89 (perhaps 86 or 87 on theirs)

"We have found a brand new HPV type for every 10 people that we have
looked at. Philodelius and Ethel Michelle Diveres and zur Hausen and Shamen
in European study of tutanius papilloma viruses have found a new papilloma
virus for just about every other person they have looked at when they use
the combination of nested PCR and DNA sequencing. Robbie Burke's group, Jill
Polefski's group, have very comparable experiences looking at anal
papillomas or female genital tract. It is my contention right now that
instead of 80 HPV genotypes or 150 that have been officially named, that
there probably are millions of variants, virtually a continuum. We feel that
basically everybody has their own personal micro flora, that these are
passively acquired or vertically acquired,not necessarily sexually, but
certainly possibly sexually, and that they simply are part of the human
condition as are microflora, just as we have microflora composed of bacteria
and many other viruses, and that they basically are utterly ubiquitous.

Page 101-102

R. BROKER: Well, I think one way is that in a very high percentage of
the people, there are clear dysplasia, low and high grade. All the women who
have any degree of dysplasia are also biopsied, and the inside 2
hybridization, as you can see, is showing clear effects in the tissue.
(pg98) So I don't think there's surface adventitious contaminants. These are
within, at least a fair number, if not all, are within the cells, and
causing various degrees of actual overt illness.

DR. RUSSO: I may have missed the data. Did you show the types that are
associated with high grade lesions, the HPV types? I didn't see on the
table.

DR. BROKER: Yes. Well basically, in this immense spectrum of what's
now 37 different viruses that we found, those that are most typically
associated with low and high grade dysplasia, the actual diseases, are the
higher risk types.

DR. RUSSO: So you are not suggesting that if you want to prevent
cervical cancer, we should focus on different types of the one already
identified?

DR. BROKER: Well the real problematic thing for any clinical
management, either vaccination programs or small molecule drugs, is this
absolutely exploding number of virus types. The one thing that I think is
going to -- and I commented a day or two ago that in the U.S. alone today,
there are over 250 to 300,000 people immuno-suppressed just due to organ transplants, (Pg 99)steroid use, or bone marrow transplants or AIDS. So there is an immense reservoir of particularly high risk patients.

Nonetheless, most of the diseases are still being caused by a handful of
viruses like 16, 18, 52. So I think, at least the ones we have to worry
about today, are still manageable in number."

~~~~~~~~~~~~~~~~~~~

You have to ask what else they don't know, and why it is they aren't telling us the truth. How can they say that "those" are the ones causing disease if, on the other hand, they swap around, and every time they look at someone else, they find a new variety, and varieties change in the same person?

HPV viruses... are they worse than the flu in terms of being shape-shifters? They probably don't know. What will the vaccine do? Cause ecological pressure and rapid variant shift? Who knows? They sure don't. Meanwhile we are told the vax will eliminate cancer. Ha.


So you see, what you can't see, can't hurt you. :ride:


And never mind that women given adequate micronutrients can clear HPviruses much faster than those whose diet is crap. http://cancerres.aacrjournals.org/cgi/reprint/67/12/5987 (http://cancerres.aacrjournals.org/cgi/reprint/67/12/5987)

[magical1]

Ohhhhh lordy!

Hey Hilary I watched a good vid today you have probably seen but others may like to see it.. It's kind of like vaccines 101.

But the point is "FALSIS IN UNO, FLASIS IN OMNIBUS"

Untrue in one thing, untrue in everything

http://video.google.com/videoplay?docid=-3859678777758345055&hl=en